Welcome to use Real-time PCR Miner for your research. Need help? , Please fill out the form below and submit your analysis request. Enjoy! Fields with "***" ahead can not be empty!

How to quantify the data by using the average efficiency of each gene and the average CT of replicates generated by Miner?

MaxP-value:  MinEfficiency:  MaxEfficiency:

***Your Email please:

***Real-time PCR platform please:

***Copy/Paste your data with sample names ( "GeneName_ReplicateName_ReplicateNumber" ) at the first row for all reactions and using a "tab" key as a seperator between reactions in each row. Please do not contain any character except all letters, numbers, and "_" in the sample name. Below is an example (Need Help?):

!!!NOTE: Before you submit data or complain the results you got, PLEASE PLEASE PLEASE read the HELP, FAQ, and the Example Excel file carefully to make sure the data are pure raw data in correct format without blank samples! Otherwise, the results might be meaningless.

!!!For the data more than 255 samples (e.g. 384 well plate), Excel can not hold all of them by column. Please copy or export them into Excel by row or simply use txt file. Thanks.

Sample by Column Sample by Row

Result by Column Result by Row