Welcome to use Real-time PCR Miner for your research. Need help? , Please fill out the form below and submit your analysis request. Enjoy! Fields with "***" ahead can not be empty!
How to quantify the data by using the average efficiency of each gene and the average CT of replicates generated by Miner?
MaxP-value: MinEfficiency: MaxEfficiency:
***Your Email please:
***Real-time PCR platform please:
ABI 7500 ABI 7700 ABI 7900 Biocompare Mx3000P BioRad iCycler BioRad MyIQ Corbett Research Rotor-Gene MJ Research DNA Engine Opticon MJ Research DNA Engine Opticon 2 MJ Research DNA Engine Opticon 4 Roche LightCycler Stratagen Mx3000 Stratagen Mx4000 SmartCycler GeneXpert Other
***Copy/Paste your data with sample names ( "GeneName_ReplicateName_ReplicateNumber" ) at the first row for all reactions and using a "tab" key as a seperator between reactions in each row. Please do not contain any character except all letters, numbers, and "_" in the sample name. Below is an example (Need Help?):
!!!NOTE: Before you submit data or complain the results you got, PLEASE PLEASE PLEASE read the HELP, FAQ, and the Example Excel file carefully to make sure the data are pure raw data in correct format without blank samples! Otherwise, the results might be meaningless.
Sample by Column Sample by Row
Result by Column Result by Row
cycle Actin_Skin_1 Actin_Skin_2 Actin_Eye_1 Actin_Eye_2 0.61 534.27 557.6 541.66 539.63 1.65 550.34 566.06 544.65 546.09 2.65 554.06 570.75 550.78 549.77 3.65 561.89 570.67 550.85 553.44 4.65 563.37 571.48 550.92 553.42 5.65 566.17 572.93 551.54 552.53 6.65 569.21 570.21 552.7 551.96 7.65 567.6 570.67 550.33 555.05 8.65 569.05 569.38 551.33 550.75 9.65 567.51 569.77 548.96 552.34 10.65 568.35 567.61 553.46 555.01 11.65 568.69 568.12 553.35 553.07 12.65 568.31 565.27 550.89 550.89 13.65 566.93 566.16 552.95 549.27 14.65 567.88 566.92 552.59 550.46 15.65 568.13 565.09 553.44 552.31 16.65 569.75 567.97 556.35 554.15 17.65 573.34 572.36 561.71 561.4 18.65 583.71 583.66 572.37 573.46 19.65 602.9 603.98 595.23 595.53 20.65 640.53 643.95 640.86 644.59 21.63 708.44 713.74 726.09 733.4 22.63 828.74 841.06 864.72 878.27 23.61 998.17 1031.37 1057.07 1075.22 24.59 1175.63 1221.34 1241.13 1260.87 25.59 1344.46 1397.14 1412.37 1433.58 26.59 1519.88 1585.85 1579.1 1602 27.58 1677.29 1754.14 1727.87 1746.41 28.58 1790.52 1877.15 1823.33 1849.57 29.58 1874.8 1963.42 1890.84 1911.73 30.58 1940.35 2026.96 1936.29 1958.83 31.55 1981.9 2064.26 1976.69 1993.51 32.55 2012.35 2097.96 2000.82 2029.69 33.58 2037.77 2130.54 2023.38 2051.42 34.58 2067.01 2153.89 2047.59 2067.47 35.58 2084.99 2166.97 2050.33 2078.97 36.58 2098.72 2185.5 2067.41 2088.14 37.55 2114 2206.62 2075.83 2101.17 38.55 2124.05 2206.75 2086.5 2108.17 39.58 2135.77 2218.09 2090.63 2111.27 40.58 2149.5 2221.74 2087.33 2122.73 41.58 2156.12 2233.86 2095.8 2130.98 42.58 2160.65 2237.15 2098.98 2133.05 43.58 2168.61 2241.77 2098.18 2134.21 44.58 2179.26 2249.29 2105.88 2142.52